Fluorescence detection is a natural luminescence reaction. By reacting luciferase with ATP, human cells, bacteria, mold, and food residues can be detected, and the reaction result is obtained within 15 seconds. The fluorescent excitation source must be capable of generating a fluorophore of sufficient intensity to absorb light waves within the band. Up to now, UVA light sources may include nitrogen molecular lasers, helium cadmium lasers, high pressure cadmium lamps, high pressure xenon lamps and metal halide arc lamps.
UVLED fluorescence detection can be integrated with the original media of the system itself. As the circulation or flow of the system medium penetrates outside the system and remains at the leak point, the probe light is probed outside the system, and the leak point emits very bright fluorescence, indicating the exact leak point of the system. Fluorescent ink anti-counterfeiting printing technology is a method of using fluorescent ink to print tickets or documents to prevent counterfeiting. The fluorescent ink can exhibit the same fluorescence in a certain part of the printed matter under the illumination of the violet light. After the fluorescent light is irradiated by the violet light, the light-emitting time is short, and the light disappears very quickly. After the purple light is turned off, the light is immediately illuminated. disappear.
UVLED fluorescence detection works by illuminating certain compounds with ultraviolet light to fluoresce and measuring the emitted fluorescence energy to quantify. Many substances related to life sciences, such as amino acids, amines, vitamins, steroids and certain metabolic drugs, can be detected by fluorescence. Fluorescence detectors are useful for trace analysis of biological samples, especially after the use of fluorescent derivatizing agents, to detect very small amounts of amino acids and peptides.
UVLED fluorescence detection applications: PCB field: exposure, curing, printing, drying FPD field: exposure, curing, printing, cleaning semiconductor field: graphics transfer, curing, printing, exposure, cleaning printing and printing areas: drying, curing, Printing, exposure, cleaning food water treatment areas: sterilization, deoxidation, TOC decomposition, food preservation medical biochemical field: sterilization, phototherapy, air cleaning, isotope separation, genetic engineering furniture apparel field: drying, curing, drying special effects screening Field: Screen screening, special effects lighting detection field: fluorescence analysis, anti-counterfeiting detection, cultural relic identification.
UVLED fluorescence detection advantages:
1 High sensitivity, UVLED fluorescence detection is the most sensitive liquid chromatography detector, especially suitable for trace analysis.
2 good selectivity. The relatively small number of molecules with large enough quantum efficiency for detection is the main reason for the high selectivity of the fluorescence detector. In many cases, the high selectivity of UVLED fluorescence detection avoids interference from non-fluorescent components and is a unique advantage of fluorescence detection.
3 linear range is wider. When the concentration of the analyte is large, the fluorescence detection intensity of the emitted UVLED may decrease as the concentration increases due to the internal filtration effect.
4 is less affected by external conditions.
5 As long as the solvent selected as the mobile phase does not emit fluorescence, UVLED fluorescence detection can be applied to gradient elution.