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UV application

Wound disinfection healing

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Shenzhen LAMPLIC CO., LTD introduced UVLED wound treatment technology from abroad, breaking through new technology in medical ultraviolet wound healing treatment. A kind of UVLED technology that uses special wound treatment UV glue to make wound cocoon quickly.
The treatment of wounds has always been an important topic in medicine. In the ancient times when medical technology was not developed, people even used horrible methods such as burning iron to treat wounds to avoid infection. In today's highly developed medical technology, surgical wounds usually need to be treated by suture or anastomosis so that the wounds can heal. For the operation of internal organs of human body, because of the presence of body fluids, the treatment of wounds will become more complex. In addition, no matter the way of suture or anastomosis, the tissue around the wound actually causes secondary injury, which is not conducive to the effective healing of the wound.
A light-curable hydrogel developed by Northeastern University, Harvard Medical College, MIT and Sydney University in Australia can close wounds within 60 seconds without affecting the contraction and relaxation of organs. It can also be natural. Degradation, and this degradation time can be adjusted according to the type of wound. There will be no residue in the body after degradation. Let's start with a video of Professor Anthony S. Weiss at the University of Sydney introducing the new findings.
This new type of photo-curable hydrogel is a methacrylated Tropoelastin, short for MeTro. Compared with the existing commercial medical sealant, MeTro has excellent adhesion and mechanical properties. Once it contacts with the tissue surface, it will become gelatinous without loss, and then further stabilized by ultraviolet irradiation. This feature allows MeTro to be precisely placed where needed and tightly connected to the structure of the tissue surface and locked into each other.
Professor Anthony S. Weiss said, "The potential applications of this product are very powerful - including dealing with serious internal injuries caused by car accidents and war zones, and improving the effectiveness of hospital surgery." "The next phase of this technology will be clinical trials. MeTro works under a range of different settings and can solve problems that other commercial medical sealants cannot solve. We are now ready to transfer our research to humans for testing. I hope that MeTro can be used in clinical practice very soon, so as to save more lives. Elastagen is commercializing the technology.
Now let's look at this amazing medical UV glue MeTro from a chemist's point of view. The golden rule of elastic tissue engineering materials is that materials must be modular, highly elastic and biocompatible polymers based on human proteins, which can be embedded by a variety of cells and can proliferate on the surface of materials. There are many kinds of elastic materials available, such as polysiloxane, polyurethane, polyhydroxyalkanoate, poly (citric acid glycol ester), poly (sebacic acid glyceride) and hybrid alginate/polyacrylamide. But most synthetic chemical elastic materials have some limitations. For example, high temperature and biotoxic reagents may be needed in the synthesis process, making them incompatible with possible cell coating. MeTro uses a bionic approach, using new engineering elastic materials derived from elastin, which widely exists in human elastic tissues. For example, elastin is found in human blood vessels, skin, heart, bladder and elastic cartilage. Elastoprotein is the natural monomer precursor of elastin. Therefore, if the recombinant elastoprotein can form protein tissue by photocrosslinking in one minute, it will bring equivalent elastic tissue.
Most elastin in the human body is produced by the slow cross-linking of elastin in uterus by the specific oxidation of lysine by lysyl oxidase. Various cross-linking methods have been used to produce three-dimensional elastin-based hydrogels from recombinant human elastin, alpha-elastin and elastin-like polypeptides. In general, these hydrogels have good biocompatibility and the ability to support cell growth in vitro and in vivo. However, low mechanical properties and heterogeneous cell infiltration into these hydrogels are problematic. In addition, chemical crosslinking agents, organic solvents, prolonged ultraviolet irradiation and high pressure were used in the preparation of these hydrogels, which made it impossible to carry out practical cell embedding during the formation of hydrogels. The synthetic route used here is photoinduced crosslinking of methacrylate-esterified elastoprotein in a short time, resulting in a highly elastic three-dimensional loadable cell hydrogel based on elastoprotein. The elastin used contains 35 lysine residues per molecule. The physical properties of the elastic hydrogels, including pore properties, swelling properties and mechanical properties, can be controlled by changing the esterification degree of methacrylic acid and polymer concentration.
The synthetic elastin of methacrylate esterified elastin (MeTro) was dissolved in PBS (10% w/v). The elastin was esterified by adding methacrylic anhydride (MA) at 4 C for 12 hours. Then the solution was dialyzed in distilled water at 4 C for 48 hours, and then lyophilized to obtain methacrylated elastin (MeTro). The products with different esterification degree can be obtained by using different concentration of methacrylic anhydride (e.g. 8, 15, 20%).
Preparation of hydrogels for performance testing MeTro macromonomers with different esterification degrees of methacrylic acid were used to prepare photocrosslinked MeTro hydrogels. Different concentrations of MeTro's PBS solution (e.g. 5,10,15% w/v) were added with 0.5% photoinitiator Irgacure 2959 (chemical name: 1-[4-(2-hydroxyethoxy) -phenyl]-2-hydroxy-2-methylacetone) at 4 ~C. The solution was placed between two slides separated by 150 micron spacers, and then irradiated with 35 seconds by using the 6.9mW/cm UV light (360 - 480 nm), and then the MeTro crosslinked MeTro gel was obtained.
The synthesized MeTro hydrogel has controllable physical properties and can be used in three-dimensional elastic environment of cell embedding and two-dimensional film of cell attachment and growth. These hydrogels can be fabricated into various shapes and sizes and exhibit the following advantages different from other hydrogels derived from elastin and synthetic elastomers:
1. MeTro gel can be rapidly formed in 35 seconds, which is much shorter than the average time needed for chemical crosslinking of elastin based hydrogels (about 24 hours).
2. photo crosslinking of MeTro gel in aqueous solution can eliminate the use of toxic coagent and organic solvents in other hydrogels.
3. The potential of rapid cell encapsulation through MeTro enables cells to disperse significantly into the matrix and overcome the problem of minimal cell penetration into the three-dimensional matrix caused by common problems such as low porosity, poor pore connectivity and small pore size.
4. unlike polyurethane and other synthetic elastomers, the biodegradable MeTro hydrogel does not cause biotoxicity in vivo degradation, because this gel is obtained from human protein.
5. Elastoprotein-based materials are more stable than other biodegradable elastomers such as PGS, which provides mechanical support before the formation of new tissues.
6. the complete secretion of elastin amino acid sequence, including the presence of C- terminal with cell interaction ability, provides the MeTro gel with endogenous integrin alpha V beta 3 cell binding site, giving it a cell interaction function.
Some mechanical properties of MeTro hydrogel materials with different esterification degree of methacrylic acid were synthesized by this method. The human protein-based hydrogel MeTro materials can be loaded on cells. They have excellent properties, including high ductility, stress and recovery after removal, as well as rapid polymerization ability and synthesis method. It is easy and suitable for three-dimensional cell embedding and two-dimensional cell implantation. These characteristics make MeTro a unique multi-functional tissue engineering material for elasticity and cell embedding and surface growth. It is believed that once this product is commercialized, it will bring a great revolution to wound repair and clinical surgery.
References https://sydney.edu.au/news-opinion/news/2017/10/05/_squirtable_-elastic-surgical-glue-seals-wounds-in-60-seconds.html N.Annabi, S.M. Mithieux, P. Zorlutuna, G. Camci-Unal, A.S. Weiss, A.Khademseini, Engineered cell-laden human protein-based Biomer.34, 5496-5505. Nasim Annabi, Yi-Nan Zhang, Alexander Assmann, Ehsan Shirzaei Sani, George Cheng, Antonio D. Lassaletta, Andrea Vegh, Bijan Dehghani, Guillermo U. Ruiz-Esparza, Xichi Wang, Sidhu Gangadharan, Anthony S. Weiss, Ali Khademhossei, Engeringa highly elastic human protein-based sealant for surgical purposes Applications, Science Translational Medicine 9, 7466-7479.
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